{"id":1225,"date":"2022-03-31T14:22:27","date_gmt":"2022-03-31T13:22:27","guid":{"rendered":"https:\/\/research.ibio.hu\/16s-amplicon-sequencing-full-length\/"},"modified":"2022-03-31T16:38:51","modified_gmt":"2022-03-31T15:38:51","slug":"16s-amplicon-sequencing-full-length","status":"publish","type":"page","link":"https:\/\/odine.hu\/en\/application-fields\/metagenomics\/16s-amplicon-sequencing-full-length\/","title":{"rendered":"16S Amplicon Sequencing (full length)"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-bg&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;179656&#8243; el_class=&#8221;page-service&#8211;content&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;-3&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; radius=&#8221;sm&#8221; width=&#8221;2\/3&#8243; uncode_shortcode_id=&#8221;200884&#8243; back_color_type=&#8221;uncode-palette&#8221; el_class=&#8221;column-main&#8221;][vc_column_text uncode_shortcode_id=&#8221;115107&#8243;]Environmental metagenomics involves the genetic analysis of microorganisms in various environmental samples. These studies provide insight into the microbiological profile of water or soil samples, allow phylogenetic analysis of microbial groups, and identify individual species.<br \/>\n16S rRNA is found in all bacteria and archaea, making it an ideal target for sequence-based identification of these organisms, especially due to the presence of conserved and variable regions in mixed samples. 16S rRNA sequencing is a cost-effective approach in environmental metagenomics.[\/vc_column_text][vc_custom_heading text_color=&#8221;color-pc&#8221; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;700&#8243; separator=&#8221;yes&#8221; uncode_shortcode_id=&#8221;834712&#8243; text_color_type=&#8221;uncode-palette&#8221;]Sample Requirements[\/vc_custom_heading][uncode_list icon=&#8221;fa fa-check2&#8243; icon_color=&#8221;color-152074&#8243; uncode_shortcode_id=&#8221;124697&#8243; el_class=&#8221;module-list&#8221; icon_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<ul>\n<li>Recommended: genomic DNA, \u2265200 ng, 20 \u00b5l, not fragmented<\/li>\n<li>Minimum: 100 ng, 15 \u00b5l<\/li>\n<\/ul>\n<p>[\/uncode_list][vc_custom_heading text_color=&#8221;color-pc&#8221; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;700&#8243; separator=&#8221;yes&#8221; uncode_shortcode_id=&#8221;104203&#8243; text_color_type=&#8221;uncode-palette&#8221;]Sequencing Strategy[\/vc_custom_heading][uncode_list icon=&#8221;fa fa-check2&#8243; icon_color=&#8221;color-152074&#8243; uncode_shortcode_id=&#8221;201840&#8243; el_class=&#8221;module-list&#8221; icon_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<ul>\n<li>1500 bp insert library<\/li>\n<li>\u226550,000 sequences<\/li>\n<\/ul>\n<p>[\/uncode_list][vc_single_image media=&#8221;1204&#8243; media_width_percent=&#8221;100&#8243; uncode_shortcode_id=&#8221;107113&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; sticky=&#8221;yes&#8221; width=&#8221;1\/3&#8243; uncode_shortcode_id=&#8221;117313&#8243; el_class=&#8221;column-menu&#8221;][vc_wp_custommenu nav_menu=&#8221;32&#8243; el_class=&#8221;module-menu&#8221;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-bg&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;179656&#8243; el_class=&#8221;page-service&#8211;content&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1260,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":""},"class_list":["post-1225","page","type-page","status-publish","hentry","page_category-metagenomics","page_category-services"],"_links":{"self":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1225","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/comments?post=1225"}],"version-history":[{"count":0,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1225\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1260"}],"wp:attachment":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/media?parent=1225"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}