{"id":1234,"date":"2022-03-31T14:29:14","date_gmt":"2022-03-31T13:29:14","guid":{"rendered":"https:\/\/research.ibio.hu\/shotgun-sequencing\/"},"modified":"2022-03-31T14:29:14","modified_gmt":"2022-03-31T13:29:14","slug":"shotgun-sequencing","status":"publish","type":"page","link":"https:\/\/odine.hu\/en\/application-fields\/metagenomics\/shotgun-sequencing\/","title":{"rendered":"Shotgun Sequencing"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-bg&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;179656&#8243; el_class=&#8221;page-service&#8211;content&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;-3&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; radius=&#8221;sm&#8221; width=&#8221;2\/3&#8243; uncode_shortcode_id=&#8221;200884&#8243; back_color_type=&#8221;uncode-palette&#8221; el_class=&#8221;column-main&#8221;][vc_column_text uncode_shortcode_id=&#8221;125381&#8243;]Environmental metagenomics involves the genetic analysis of microorganisms in various environmental samples. These studies provide insight into the microbiological profile of water or soil samples, allow phylogenetic analysis of microbial groups, and identify individual species.<\/p>\n<p>Complete (shotgun) metagenome sequencing provides information on the entire genome of all organisms without bias. In addition to information on taxonomic classification and relative abundance of organisms without isolation and culture, this technique is invaluable in studying gene prediction, functional profiling and the interaction between microbial species.[\/vc_column_text][vc_custom_heading text_color=&#8221;color-pc&#8221; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;700&#8243; separator=&#8221;yes&#8221; uncode_shortcode_id=&#8221;834712&#8243; text_color_type=&#8221;uncode-palette&#8221;]Sample Requirements[\/vc_custom_heading][uncode_list icon=&#8221;fa fa-check2&#8243; icon_color=&#8221;color-152074&#8243; uncode_shortcode_id=&#8221;113298&#8243; el_class=&#8221;module-list&#8221; icon_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<ul>\n<li>Recommended: genomic DNA, \u2265600 ng, 20 \u00b5l, not fragmented<\/li>\n<li>Minimum: 150 ng, 15 \u00b5l<\/li>\n<\/ul>\n<p>[\/uncode_list][vc_custom_heading text_color=&#8221;color-pc&#8221; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;700&#8243; separator=&#8221;yes&#8221; uncode_shortcode_id=&#8221;104203&#8243; text_color_type=&#8221;uncode-palette&#8221;]Sequencing Strategy[\/vc_custom_heading][uncode_list icon=&#8221;fa fa-check2&#8243; icon_color=&#8221;color-152074&#8243; uncode_shortcode_id=&#8221;167644&#8243; el_class=&#8221;module-list&#8221; icon_color_type=&#8221;uncode-palette&#8221;]<\/p>\n<ul>\n<li>250-300 bp insert library<\/li>\n<li>Paired-end 150 bp sequencing<\/li>\n<li>\u22656 Gbp data<\/li>\n<\/ul>\n<p>[\/uncode_list][vc_single_image media=&#8221;1198&#8243; media_width_percent=&#8221;100&#8243; uncode_shortcode_id=&#8221;947292&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; sticky=&#8221;yes&#8221; width=&#8221;1\/3&#8243; uncode_shortcode_id=&#8221;117313&#8243; el_class=&#8221;column-menu&#8221;][vc_wp_custommenu nav_menu=&#8221;32&#8243; el_class=&#8221;module-menu&#8221;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-bg&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;179656&#8243; el_class=&#8221;page-service&#8211;content&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1260,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":""},"class_list":["post-1234","page","type-page","status-publish","hentry","page_category-metagenomics","page_category-services"],"_links":{"self":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1234","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/comments?post=1234"}],"version-history":[{"count":0,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1234\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1260"}],"wp:attachment":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/media?parent=1234"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}