{"id":1250,"date":"2022-03-31T14:39:37","date_gmt":"2022-03-31T13:39:37","guid":{"rendered":"https:\/\/research.ibio.hu\/dns-sequensinc\/whole-genome-sequencing-long-sequences\/"},"modified":"2022-03-31T16:38:36","modified_gmt":"2022-03-31T15:38:36","slug":"whole-genome-sequencing-long-sequences","status":"publish","type":"page","link":"https:\/\/odine.hu\/en\/application-fields\/dns-sequensinc\/whole-genome-sequencing-long-sequences\/","title":{"rendered":"Whole genome sequencing (long sequences)"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-bg&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;137948&#8243; el_class=&#8221;page-service&#8211;content&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;-3&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; radius=&#8221;sm&#8221; width=&#8221;2\/3&#8243; uncode_shortcode_id=&#8221;200884&#8243; back_color_type=&#8221;uncode-palette&#8221; el_class=&#8221;column-main&#8221;][vc_column_text uncode_shortcode_id=&#8221;336679&#8243;]Next-generation sequencing (NGS) methods can be used to determine the DNA sequence of any organism, providing valuable information to answer almost any biological question.<\/p>\n<p>NGS is highly scalable and can be applied to small, targeted regions or the entire genome. NGS is highly scalable and can be applied to small, targeted regions or to the entire genome. Whole genome sequencing (WGS) is a comprehensive method for analyzing the entire genome of not only humans but any species, such as agriculturally important livestock, plants or disease-related microbes.[\/vc_column_text][vc_single_image media=&#8221;1150&#8243; media_width_percent=&#8221;100&#8243; uncode_shortcode_id=&#8221;116356&#8243;][vc_custom_heading text_color=&#8221;color-pc&#8221; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;700&#8243; separator=&#8221;yes&#8221; uncode_shortcode_id=&#8221;430315&#8243; text_color_type=&#8221;uncode-palette&#8221;]Sample Requirements[\/vc_custom_heading][uncode_list icon=&#8221;fa fa-check2&#8243; uncode_shortcode_id=&#8221;201734&#8243; el_class=&#8221;module-list&#8221; icon_color_type=&#8221;uncode-solid&#8221; icon_color_solid=&#8221;#006fd1&#8243;]<\/p>\n<ul>\n<li>Recommended: genomic DNA, \u22652000 ng, 50 \u00b5l, not fragmented<\/li>\n<li>Minimum: 1000 ng, 15 \u00b5l<\/li>\n<\/ul>\n<p>[\/uncode_list][vc_custom_heading text_color=&#8221;color-pc&#8221; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;700&#8243; separator=&#8221;yes&#8221; uncode_shortcode_id=&#8221;171740&#8243; text_color_type=&#8221;uncode-palette&#8221;]Sequencing Strategy[\/vc_custom_heading][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;2&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; limit_content=&#8221;&#8221; uncode_shortcode_id=&#8221;192933&#8243;][vc_column_inner width=&#8221;2\/3&#8243;][uncode_list icon=&#8221;fa fa-check2&#8243; uncode_shortcode_id=&#8221;146272&#8243; el_class=&#8221;module-list&#8221; icon_color_type=&#8221;uncode-solid&#8221; icon_color_solid=&#8221;#006fd1&#8243;]<\/p>\n<ul>\n<li>20-30 Kbp insert library<\/li>\n<li>\u226530x coverage<\/li>\n<\/ul>\n<p>[\/uncode_list][\/vc_column_inner][\/vc_row_inner][vc_single_image media=&#8221;1208&#8243; media_width_percent=&#8221;100&#8243; uncode_shortcode_id=&#8221;148491&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; sticky=&#8221;yes&#8221; width=&#8221;1\/3&#8243; uncode_shortcode_id=&#8221;117313&#8243; el_class=&#8221;column-menu&#8221;][vc_wp_custommenu nav_menu=&#8221;30&#8243; el_class=&#8221;module-menu&#8221;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-bg&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;137948&#8243; el_class=&#8221;page-service&#8211;content&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1223,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":""},"class_list":["post-1250","page","type-page","status-publish","hentry","page_category-dna-sequencing","page_category-services"],"_links":{"self":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1250","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/comments?post=1250"}],"version-history":[{"count":0,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1250\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1223"}],"wp:attachment":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/media?parent=1250"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}