{"id":1256,"date":"2022-03-31T14:41:38","date_gmt":"2022-03-31T13:41:38","guid":{"rendered":"https:\/\/research.ibio.hu\/dns-sequensinc\/whole-genome-sequencing-short-sequences\/"},"modified":"2022-03-31T16:38:36","modified_gmt":"2022-03-31T15:38:36","slug":"whole-genome-sequencing-short-sequences","status":"publish","type":"page","link":"https:\/\/odine.hu\/en\/application-fields\/dns-sequensinc\/whole-genome-sequencing-short-sequences\/","title":{"rendered":"Whole genome sequencing (short sequences)"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-bg&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;137948&#8243; el_class=&#8221;page-service&#8211;content&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;-3&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; radius=&#8221;sm&#8221; width=&#8221;2\/3&#8243; uncode_shortcode_id=&#8221;200884&#8243; back_color_type=&#8221;uncode-palette&#8221; el_class=&#8221;column-main&#8221;][vc_column_text uncode_shortcode_id=&#8221;140625&#8243;]Next-generation sequencing (NGS) methods can be used to determine the DNA sequence of any organism, providing valuable information to answer almost any biological question.<\/p>\n<p>NGS is highly scalable and can be applied to small, targeted regions or to the entire genome. Whole genome sequencing (WGS) is a comprehensive method for analyzing the entire genome of not only humans but any species, such as agriculturally important livestock, plants, or disease-related microbes.[\/vc_column_text][vc_single_image media=&#8221;1152&#8243; media_width_percent=&#8221;100&#8243; uncode_shortcode_id=&#8221;221686&#8243;][vc_custom_heading text_color=&#8221;color-pc&#8221; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;700&#8243; separator=&#8221;yes&#8221; uncode_shortcode_id=&#8221;430315&#8243; text_color_type=&#8221;uncode-palette&#8221;]Sample Requirements[\/vc_custom_heading][uncode_list icon=&#8221;fa fa-check2&#8243; uncode_shortcode_id=&#8221;209148&#8243; el_class=&#8221;module-list&#8221; icon_color_type=&#8221;uncode-solid&#8221; icon_color_solid=&#8221;#006fd1&#8243;]<\/p>\n<ul>\n<li>Recommended: genomic DNA, \u2265600 ng, 20 \u00b5l, not fragmented<\/li>\n<li>Minimum: 200 ng, 15 \u00b5l<\/li>\n<\/ul>\n<p>[\/uncode_list][vc_custom_heading text_color=&#8221;color-pc&#8221; heading_semantic=&#8221;h4&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;700&#8243; separator=&#8221;yes&#8221; uncode_shortcode_id=&#8221;171740&#8243; text_color_type=&#8221;uncode-palette&#8221;]Sequencing Strategy[\/vc_custom_heading][vc_row_inner row_inner_height_percent=&#8221;0&#8243; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;2&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; limit_content=&#8221;&#8221; uncode_shortcode_id=&#8221;192933&#8243;][vc_column_inner width=&#8221;2\/3&#8243;][uncode_list icon=&#8221;fa fa-check2&#8243; uncode_shortcode_id=&#8221;581821&#8243; el_class=&#8221;module-list&#8221; icon_color_type=&#8221;uncode-solid&#8221; icon_color_solid=&#8221;#006fd1&#8243;]<\/p>\n<ul>\n<li>250-350 bp insert library<\/li>\n<li>Paired-end 150 bp sequencing<\/li>\n<li>\u22651000x coverage<\/li>\n<\/ul>\n<p>[\/uncode_list][\/vc_column_inner][\/vc_row_inner][vc_single_image media=&#8221;1198&#8243; media_width_percent=&#8221;100&#8243; uncode_shortcode_id=&#8221;144716&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; sticky=&#8221;yes&#8221; width=&#8221;1\/3&#8243; uncode_shortcode_id=&#8221;117313&#8243; el_class=&#8221;column-menu&#8221;][vc_wp_custommenu nav_menu=&#8221;30&#8243; el_class=&#8221;module-menu&#8221;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;2&#8243; top_padding=&#8221;2&#8243; bottom_padding=&#8221;3&#8243; back_color=&#8221;color-bg&#8221; overlay_alpha=&#8221;50&#8243; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; inverted_device_order=&#8221;yes&#8221; uncode_shortcode_id=&#8221;137948&#8243; el_class=&#8221;page-service&#8211;content&#8221; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;3&#8243; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1223,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":""},"class_list":["post-1256","page","type-page","status-publish","hentry","page_category-dna-sequencing","page_category-services"],"_links":{"self":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1256","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/comments?post=1256"}],"version-history":[{"count":0,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1256\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/pages\/1223"}],"wp:attachment":[{"href":"https:\/\/odine.hu\/en\/wp-json\/wp\/v2\/media?parent=1256"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}